fos 2a icreer Search Results


trap2 fos 2a icreer  (Jackson Laboratory)
86
Jackson Laboratory trap2 fos 2a icreer
Trap2 Fos 2a Icreer, supplied by Jackson Laboratory, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/fos+2a+icreer/fos2a+icreer/pmc13201567-464-0-15
Average 86 stars, based on 1 article reviews
trap2 fos 2a icreer - by Bioz Stars, 2026-09
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176 animals 177 fos  (Jackson Laboratory)
86
Jackson Laboratory 176 animals 177 fos
176 Animals 177 Fos, supplied by Jackson Laboratory, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/fos+2a+icreer/2a+fos+icreert2/10__1523_slash_eneuro__0400___25__2026-50-32-38
Average 86 stars, based on 1 article reviews
176 animals 177 fos - by Bioz Stars, 2026-09
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trap2  (Jackson Laboratory)
86
Jackson Laboratory trap2
Trap2, supplied by Jackson Laboratory, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/fos+2a+icreer/mice+trap2/pmc10922047-506-0-3
Average 86 stars, based on 1 article reviews
trap2 - by Bioz Stars, 2026-09
86/100 stars
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fostrap  (Jackson Laboratory)
86
Jackson Laboratory fostrap
A , Mechanism of activity-dependent GFP expression in tagged neurons and tdTomato (tdT) expression in dMSNs of <t>FosTRAP;Snap25-GFP;D1-tdT</t> mice. B , Behavioral and tagging timeline illustrating acquisition, extinction or no-extinction, reinstatement testing, and tissue collection for ensemble reactivation analysis using c-Fos immunohistochemistry (IHC). dMSNs express tdT. After tagging, tagged dMSNs (dMSN tag ) co-express Snap25-GFP and D1-tdT. During reinstatement, reactivated dMSN tag were labeled with c-Fos IHC. C , Active lever pressing during final FR3 session and reinstatement in extinction and no-extinction groups. Two-way RM ANOVA: F (5, 20) = 4.981, ## p=0.0040; Sidak’s multiple comparisons test for No-Ext vs Ext: Acq sessions 1-5: t = 1.24, 0.74, 0.083, 0.85, 1.51, p = 0.78, 0.98, 0.99, 0.95, 0.61; Rein: t = 6.318, ***p < 0.001. D , Representative confocal images of dorsomedial striatum showing tagged acquisition neurons (GFP), D1-tdT labeling, and c-Fos expression following reinstatement. Insets show higher magnification views. E , Both groups had similar densities of tagged neurons in the dorsomedial striatum. Unpaired t test: t (24) = 1.80, p = 0.08. F , Both groups had similar densities of dMSN tag . Unpaired t test: t (24) = 1.18, p = 0.25. G , Extinction training reduced the density of reactivated dMSN tag during reinstatement. Unpaired t test: t (24) = 7.43, ***p < 0.001. H , Extinction training reduced the percentage of acquisition-recruited dMSNs reactivated during reinstatement. Unpaired t test: t (24) = 6.33, ***p < 0.001. n = 3 mice per group (C), 12 slices/3 mice (No-Ext; E-H) and 14 slices/3 mice (Ext; E-H).
Fostrap, supplied by Jackson Laboratory, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/fos+2a+icreer/fostrap+mice/bio_rxiv__64898__2026__04__30__722024-136-4-21
Average 86 stars, based on 1 article reviews
fostrap - by Bioz Stars, 2026-09
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tm2 1  (Jackson Laboratory)
86
Jackson Laboratory tm2 1
A , Mechanism of activity-dependent GFP expression in tagged neurons and tdTomato (tdT) expression in dMSNs of <t>FosTRAP;Snap25-GFP;D1-tdT</t> mice. B , Behavioral and tagging timeline illustrating acquisition, extinction or no-extinction, reinstatement testing, and tissue collection for ensemble reactivation analysis using c-Fos immunohistochemistry (IHC). dMSNs express tdT. After tagging, tagged dMSNs (dMSN tag ) co-express Snap25-GFP and D1-tdT. During reinstatement, reactivated dMSN tag were labeled with c-Fos IHC. C , Active lever pressing during final FR3 session and reinstatement in extinction and no-extinction groups. Two-way RM ANOVA: F (5, 20) = 4.981, ## p=0.0040; Sidak’s multiple comparisons test for No-Ext vs Ext: Acq sessions 1-5: t = 1.24, 0.74, 0.083, 0.85, 1.51, p = 0.78, 0.98, 0.99, 0.95, 0.61; Rein: t = 6.318, ***p < 0.001. D , Representative confocal images of dorsomedial striatum showing tagged acquisition neurons (GFP), D1-tdT labeling, and c-Fos expression following reinstatement. Insets show higher magnification views. E , Both groups had similar densities of tagged neurons in the dorsomedial striatum. Unpaired t test: t (24) = 1.80, p = 0.08. F , Both groups had similar densities of dMSN tag . Unpaired t test: t (24) = 1.18, p = 0.25. G , Extinction training reduced the density of reactivated dMSN tag during reinstatement. Unpaired t test: t (24) = 7.43, ***p < 0.001. H , Extinction training reduced the percentage of acquisition-recruited dMSNs reactivated during reinstatement. Unpaired t test: t (24) = 6.33, ***p < 0.001. n = 3 mice per group (C), 12 slices/3 mice (No-Ext; E-H) and 14 slices/3 mice (Ext; E-H).
Tm2 1, supplied by Jackson Laboratory, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/fos+2a+icreer/1+ert2+fostm2+icre+j+luo/pmc13067151-41-2-5
Average 86 stars, based on 1 article reviews
tm2 1 - by Bioz Stars, 2026-09
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recombination  (Jackson Laboratory)
86
Jackson Laboratory recombination
A , Mechanism of activity-dependent GFP expression in tagged neurons and tdTomato (tdT) expression in dMSNs of <t>FosTRAP;Snap25-GFP;D1-tdT</t> mice. B , Behavioral and tagging timeline illustrating acquisition, extinction or no-extinction, reinstatement testing, and tissue collection for ensemble reactivation analysis using c-Fos immunohistochemistry (IHC). dMSNs express tdT. After tagging, tagged dMSNs (dMSN tag ) co-express Snap25-GFP and D1-tdT. During reinstatement, reactivated dMSN tag were labeled with c-Fos IHC. C , Active lever pressing during final FR3 session and reinstatement in extinction and no-extinction groups. Two-way RM ANOVA: F (5, 20) = 4.981, ## p=0.0040; Sidak’s multiple comparisons test for No-Ext vs Ext: Acq sessions 1-5: t = 1.24, 0.74, 0.083, 0.85, 1.51, p = 0.78, 0.98, 0.99, 0.95, 0.61; Rein: t = 6.318, ***p < 0.001. D , Representative confocal images of dorsomedial striatum showing tagged acquisition neurons (GFP), D1-tdT labeling, and c-Fos expression following reinstatement. Insets show higher magnification views. E , Both groups had similar densities of tagged neurons in the dorsomedial striatum. Unpaired t test: t (24) = 1.80, p = 0.08. F , Both groups had similar densities of dMSN tag . Unpaired t test: t (24) = 1.18, p = 0.25. G , Extinction training reduced the density of reactivated dMSN tag during reinstatement. Unpaired t test: t (24) = 7.43, ***p < 0.001. H , Extinction training reduced the percentage of acquisition-recruited dMSNs reactivated during reinstatement. Unpaired t test: t (24) = 6.33, ***p < 0.001. n = 3 mice per group (C), 12 slices/3 mice (No-Ext; E-H) and 14 slices/3 mice (Ext; E-H).
Recombination, supplied by Jackson Laboratory, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/fos+2a+icreer/recombination/bio_rxiv__64898__2026__01__28__702313-120-5-17
Average 86 stars, based on 1 article reviews
recombination - by Bioz Stars, 2026-09
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vectashield antifade mounting medium with dapi  (Vector Laboratories)
98
Vector Laboratories vectashield antifade mounting medium with dapi
A , Mechanism of activity-dependent GFP expression in tagged neurons and tdTomato (tdT) expression in dMSNs of <t>FosTRAP;Snap25-GFP;D1-tdT</t> mice. B , Behavioral and tagging timeline illustrating acquisition, extinction or no-extinction, reinstatement testing, and tissue collection for ensemble reactivation analysis using c-Fos immunohistochemistry (IHC). dMSNs express tdT. After tagging, tagged dMSNs (dMSN tag ) co-express Snap25-GFP and D1-tdT. During reinstatement, reactivated dMSN tag were labeled with c-Fos IHC. C , Active lever pressing during final FR3 session and reinstatement in extinction and no-extinction groups. Two-way RM ANOVA: F (5, 20) = 4.981, ## p=0.0040; Sidak’s multiple comparisons test for No-Ext vs Ext: Acq sessions 1-5: t = 1.24, 0.74, 0.083, 0.85, 1.51, p = 0.78, 0.98, 0.99, 0.95, 0.61; Rein: t = 6.318, ***p < 0.001. D , Representative confocal images of dorsomedial striatum showing tagged acquisition neurons (GFP), D1-tdT labeling, and c-Fos expression following reinstatement. Insets show higher magnification views. E , Both groups had similar densities of tagged neurons in the dorsomedial striatum. Unpaired t test: t (24) = 1.80, p = 0.08. F , Both groups had similar densities of dMSN tag . Unpaired t test: t (24) = 1.18, p = 0.25. G , Extinction training reduced the density of reactivated dMSN tag during reinstatement. Unpaired t test: t (24) = 7.43, ***p < 0.001. H , Extinction training reduced the percentage of acquisition-recruited dMSNs reactivated during reinstatement. Unpaired t test: t (24) = 6.33, ***p < 0.001. n = 3 mice per group (C), 12 slices/3 mice (No-Ext; E-H) and 14 slices/3 mice (Ext; E-H).
Vectashield Antifade Mounting Medium With Dapi, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 98/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/fos+2a+icreer/VECTASHIELD+Antifade+Mounting+Medium+with+DAPI/custom%40h-1200%4037881549
Average 98 stars, based on 1 article reviews
vectashield antifade mounting medium with dapi - by Bioz Stars, 2026-09
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ai9  (Jackson Laboratory)
86
Jackson Laboratory ai9
A , Mechanism of activity-dependent GFP expression in tagged neurons and tdTomato (tdT) expression in dMSNs of <t>FosTRAP;Snap25-GFP;D1-tdT</t> mice. B , Behavioral and tagging timeline illustrating acquisition, extinction or no-extinction, reinstatement testing, and tissue collection for ensemble reactivation analysis using c-Fos immunohistochemistry (IHC). dMSNs express tdT. After tagging, tagged dMSNs (dMSN tag ) co-express Snap25-GFP and D1-tdT. During reinstatement, reactivated dMSN tag were labeled with c-Fos IHC. C , Active lever pressing during final FR3 session and reinstatement in extinction and no-extinction groups. Two-way RM ANOVA: F (5, 20) = 4.981, ## p=0.0040; Sidak’s multiple comparisons test for No-Ext vs Ext: Acq sessions 1-5: t = 1.24, 0.74, 0.083, 0.85, 1.51, p = 0.78, 0.98, 0.99, 0.95, 0.61; Rein: t = 6.318, ***p < 0.001. D , Representative confocal images of dorsomedial striatum showing tagged acquisition neurons (GFP), D1-tdT labeling, and c-Fos expression following reinstatement. Insets show higher magnification views. E , Both groups had similar densities of tagged neurons in the dorsomedial striatum. Unpaired t test: t (24) = 1.80, p = 0.08. F , Both groups had similar densities of dMSN tag . Unpaired t test: t (24) = 1.18, p = 0.25. G , Extinction training reduced the density of reactivated dMSN tag during reinstatement. Unpaired t test: t (24) = 7.43, ***p < 0.001. H , Extinction training reduced the percentage of acquisition-recruited dMSNs reactivated during reinstatement. Unpaired t test: t (24) = 6.33, ***p < 0.001. n = 3 mice per group (C), 12 slices/3 mice (No-Ext; E-H) and 14 slices/3 mice (Ext; E-H).
Ai9, supplied by Jackson Laboratory, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/fos+2a+icreer/ai9/pm41781500-325-4-15
Average 86 stars, based on 1 article reviews
ai9 - by Bioz Stars, 2026-09
86/100 stars
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b6 cg  (Jackson Laboratory)
86
Jackson Laboratory b6 cg
A , Mechanism of activity-dependent GFP expression in tagged neurons and tdTomato (tdT) expression in dMSNs of <t>FosTRAP;Snap25-GFP;D1-tdT</t> mice. B , Behavioral and tagging timeline illustrating acquisition, extinction or no-extinction, reinstatement testing, and tissue collection for ensemble reactivation analysis using c-Fos immunohistochemistry (IHC). dMSNs express tdT. After tagging, tagged dMSNs (dMSN tag ) co-express Snap25-GFP and D1-tdT. During reinstatement, reactivated dMSN tag were labeled with c-Fos IHC. C , Active lever pressing during final FR3 session and reinstatement in extinction and no-extinction groups. Two-way RM ANOVA: F (5, 20) = 4.981, ## p=0.0040; Sidak’s multiple comparisons test for No-Ext vs Ext: Acq sessions 1-5: t = 1.24, 0.74, 0.083, 0.85, 1.51, p = 0.78, 0.98, 0.99, 0.95, 0.61; Rein: t = 6.318, ***p < 0.001. D , Representative confocal images of dorsomedial striatum showing tagged acquisition neurons (GFP), D1-tdT labeling, and c-Fos expression following reinstatement. Insets show higher magnification views. E , Both groups had similar densities of tagged neurons in the dorsomedial striatum. Unpaired t test: t (24) = 1.80, p = 0.08. F , Both groups had similar densities of dMSN tag . Unpaired t test: t (24) = 1.18, p = 0.25. G , Extinction training reduced the density of reactivated dMSN tag during reinstatement. Unpaired t test: t (24) = 7.43, ***p < 0.001. H , Extinction training reduced the percentage of acquisition-recruited dMSNs reactivated during reinstatement. Unpaired t test: t (24) = 6.33, ***p < 0.001. n = 3 mice per group (C), 12 slices/3 mice (No-Ext; E-H) and 14 slices/3 mice (Ext; E-H).
B6 Cg, supplied by Jackson Laboratory, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/fos+2a+icreer/b6+cg/bio_rxiv__64898__2026__03__14__711088-33-4-12
Average 86 stars, based on 1 article reviews
b6 cg - by Bioz Stars, 2026-09
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drd1a tdtomato  (Jackson Laboratory)
86
Jackson Laboratory drd1a tdtomato
A , Mechanism of activity-dependent GFP expression in tagged neurons and tdTomato (tdT) expression in dMSNs of <t>FosTRAP;Snap25-GFP;D1-tdT</t> mice. B , Behavioral and tagging timeline illustrating acquisition, extinction or no-extinction, reinstatement testing, and tissue collection for ensemble reactivation analysis using c-Fos immunohistochemistry (IHC). dMSNs express tdT. After tagging, tagged dMSNs (dMSN tag ) co-express Snap25-GFP and D1-tdT. During reinstatement, reactivated dMSN tag were labeled with c-Fos IHC. C , Active lever pressing during final FR3 session and reinstatement in extinction and no-extinction groups. Two-way RM ANOVA: F (5, 20) = 4.981, ## p=0.0040; Sidak’s multiple comparisons test for No-Ext vs Ext: Acq sessions 1-5: t = 1.24, 0.74, 0.083, 0.85, 1.51, p = 0.78, 0.98, 0.99, 0.95, 0.61; Rein: t = 6.318, ***p < 0.001. D , Representative confocal images of dorsomedial striatum showing tagged acquisition neurons (GFP), D1-tdT labeling, and c-Fos expression following reinstatement. Insets show higher magnification views. E , Both groups had similar densities of tagged neurons in the dorsomedial striatum. Unpaired t test: t (24) = 1.80, p = 0.08. F , Both groups had similar densities of dMSN tag . Unpaired t test: t (24) = 1.18, p = 0.25. G , Extinction training reduced the density of reactivated dMSN tag during reinstatement. Unpaired t test: t (24) = 7.43, ***p < 0.001. H , Extinction training reduced the percentage of acquisition-recruited dMSNs reactivated during reinstatement. Unpaired t test: t (24) = 6.33, ***p < 0.001. n = 3 mice per group (C), 12 slices/3 mice (No-Ext; E-H) and 14 slices/3 mice (Ext; E-H).
Drd1a Tdtomato, supplied by Jackson Laboratory, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/fos+2a+icreer/drd1a+tdtomato/bio_rxiv__64898__2026__04__30__722024-136-0-21
Average 86 stars, based on 1 article reviews
drd1a tdtomato - by Bioz Stars, 2026-09
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crh ires cre  (Jackson Laboratory)
86
Jackson Laboratory crh ires cre
A , Mechanism of activity-dependent GFP expression in tagged neurons and tdTomato (tdT) expression in dMSNs of <t>FosTRAP;Snap25-GFP;D1-tdT</t> mice. B , Behavioral and tagging timeline illustrating acquisition, extinction or no-extinction, reinstatement testing, and tissue collection for ensemble reactivation analysis using c-Fos immunohistochemistry (IHC). dMSNs express tdT. After tagging, tagged dMSNs (dMSN tag ) co-express Snap25-GFP and D1-tdT. During reinstatement, reactivated dMSN tag were labeled with c-Fos IHC. C , Active lever pressing during final FR3 session and reinstatement in extinction and no-extinction groups. Two-way RM ANOVA: F (5, 20) = 4.981, ## p=0.0040; Sidak’s multiple comparisons test for No-Ext vs Ext: Acq sessions 1-5: t = 1.24, 0.74, 0.083, 0.85, 1.51, p = 0.78, 0.98, 0.99, 0.95, 0.61; Rein: t = 6.318, ***p < 0.001. D , Representative confocal images of dorsomedial striatum showing tagged acquisition neurons (GFP), D1-tdT labeling, and c-Fos expression following reinstatement. Insets show higher magnification views. E , Both groups had similar densities of tagged neurons in the dorsomedial striatum. Unpaired t test: t (24) = 1.80, p = 0.08. F , Both groups had similar densities of dMSN tag . Unpaired t test: t (24) = 1.18, p = 0.25. G , Extinction training reduced the density of reactivated dMSN tag during reinstatement. Unpaired t test: t (24) = 7.43, ***p < 0.001. H , Extinction training reduced the percentage of acquisition-recruited dMSNs reactivated during reinstatement. Unpaired t test: t (24) = 6.33, ***p < 0.001. n = 3 mice per group (C), 12 slices/3 mice (No-Ext; E-H) and 14 slices/3 mice (Ext; E-H).
Crh Ires Cre, supplied by Jackson Laboratory, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/fos+2a+icreer/cre+vglut2/bio_rxiv__64898__2026__04__30__722112-282-6-27
Average 86 stars, based on 1 article reviews
crh ires cre - by Bioz Stars, 2026-09
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arctrap  (Jackson Laboratory)
86
Jackson Laboratory arctrap
A , During extinction, acquisition-recruited striatal ensembles are suppressed, contributing to the reduction of alcohol-seeking behavior. We hypothesized that chemogenetic activation of these acquisition-recruited neurons during extinction would counteract this suppression, impair extinction learning, and promote relapse. B , Schematic illustrating intersectional viral approach to express DREADD in acquisition-recruited striatal dMSNs in <t>ArcTRAP</t> mice. C , Following viral infusion, mice underwent ∼8 weeks of operant alcohol self-administration, with tagging occurred during the last two days of the training. Two weeks later, they underwent daily chemogenetic activation (via C21 injection, 1 mg/kg) during extinction sessions, followed by cue-induced reinstatement testing without C21 injection. D , Active lever pressing during acquisition was comparable between groups. Two-way RM ANOVA: F (1, 11) = 0.29, p = 0.60. E , Chemogenetic activation of acquisition-recruited dMSNs resulted in persistently elevated responding across extinction sessions. Two-way RM ANOVA: F (1, 11) = 5.697, # p = 0.036. F , Activation of acquisition-recruited dMSNs during extinction enhanced active presses during cue-induced reinstatement. Unpaired t test: t (11) = 2.46, *p = 0.032. G , Reinstatement responding expressed as a percentage of acquisition responding demonstrates that activation of acquisition ensembles during extinction promotes relapse. Unpaired t test: t (11) = 2.59, *p = 0.025. n = 7 mice (mCh) and 6 mice (Gq).
Arctrap, supplied by Jackson Laboratory, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/fos+2a+icreer/arctrap/bio_rxiv__64898__2026__04__30__722024-136-10-21
Average 86 stars, based on 1 article reviews
arctrap - by Bioz Stars, 2026-09
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Image Search Results


A , Mechanism of activity-dependent GFP expression in tagged neurons and tdTomato (tdT) expression in dMSNs of FosTRAP;Snap25-GFP;D1-tdT mice. B , Behavioral and tagging timeline illustrating acquisition, extinction or no-extinction, reinstatement testing, and tissue collection for ensemble reactivation analysis using c-Fos immunohistochemistry (IHC). dMSNs express tdT. After tagging, tagged dMSNs (dMSN tag ) co-express Snap25-GFP and D1-tdT. During reinstatement, reactivated dMSN tag were labeled with c-Fos IHC. C , Active lever pressing during final FR3 session and reinstatement in extinction and no-extinction groups. Two-way RM ANOVA: F (5, 20) = 4.981, ## p=0.0040; Sidak’s multiple comparisons test for No-Ext vs Ext: Acq sessions 1-5: t = 1.24, 0.74, 0.083, 0.85, 1.51, p = 0.78, 0.98, 0.99, 0.95, 0.61; Rein: t = 6.318, ***p < 0.001. D , Representative confocal images of dorsomedial striatum showing tagged acquisition neurons (GFP), D1-tdT labeling, and c-Fos expression following reinstatement. Insets show higher magnification views. E , Both groups had similar densities of tagged neurons in the dorsomedial striatum. Unpaired t test: t (24) = 1.80, p = 0.08. F , Both groups had similar densities of dMSN tag . Unpaired t test: t (24) = 1.18, p = 0.25. G , Extinction training reduced the density of reactivated dMSN tag during reinstatement. Unpaired t test: t (24) = 7.43, ***p < 0.001. H , Extinction training reduced the percentage of acquisition-recruited dMSNs reactivated during reinstatement. Unpaired t test: t (24) = 6.33, ***p < 0.001. n = 3 mice per group (C), 12 slices/3 mice (No-Ext; E-H) and 14 slices/3 mice (Ext; E-H).

Journal: bioRxiv

Article Title: Extinction Training Suppresses Alcohol Relapse by Inhibiting Acquisition-Recruited Striatal Ensembles and Engaging Striosomal Neurons

doi: 10.64898/2026.04.30.722024

Figure Lengend Snippet: A , Mechanism of activity-dependent GFP expression in tagged neurons and tdTomato (tdT) expression in dMSNs of FosTRAP;Snap25-GFP;D1-tdT mice. B , Behavioral and tagging timeline illustrating acquisition, extinction or no-extinction, reinstatement testing, and tissue collection for ensemble reactivation analysis using c-Fos immunohistochemistry (IHC). dMSNs express tdT. After tagging, tagged dMSNs (dMSN tag ) co-express Snap25-GFP and D1-tdT. During reinstatement, reactivated dMSN tag were labeled with c-Fos IHC. C , Active lever pressing during final FR3 session and reinstatement in extinction and no-extinction groups. Two-way RM ANOVA: F (5, 20) = 4.981, ## p=0.0040; Sidak’s multiple comparisons test for No-Ext vs Ext: Acq sessions 1-5: t = 1.24, 0.74, 0.083, 0.85, 1.51, p = 0.78, 0.98, 0.99, 0.95, 0.61; Rein: t = 6.318, ***p < 0.001. D , Representative confocal images of dorsomedial striatum showing tagged acquisition neurons (GFP), D1-tdT labeling, and c-Fos expression following reinstatement. Insets show higher magnification views. E , Both groups had similar densities of tagged neurons in the dorsomedial striatum. Unpaired t test: t (24) = 1.80, p = 0.08. F , Both groups had similar densities of dMSN tag . Unpaired t test: t (24) = 1.18, p = 0.25. G , Extinction training reduced the density of reactivated dMSN tag during reinstatement. Unpaired t test: t (24) = 7.43, ***p < 0.001. H , Extinction training reduced the percentage of acquisition-recruited dMSNs reactivated during reinstatement. Unpaired t test: t (24) = 6.33, ***p < 0.001. n = 3 mice per group (C), 12 slices/3 mice (No-Ext; E-H) and 14 slices/3 mice (Ext; E-H).

Article Snippet: Drd1a-tdTomato (#016204), Snap25-LSL-F2A-GFP (#021879), FosTRAP (Fos 2A-iCreER ; #030323), and ArcTRAP (Arc CreER ; #021881) , , mice were purchased from the Jackson Laboratory.

Techniques: Activity Assay, Expressing, Immunohistochemistry, Labeling

A , During extinction, acquisition-recruited striatal ensembles are suppressed, contributing to the reduction of alcohol-seeking behavior. We hypothesized that chemogenetic activation of these acquisition-recruited neurons during extinction would counteract this suppression, impair extinction learning, and promote relapse. B , Schematic illustrating intersectional viral approach to express DREADD in acquisition-recruited striatal dMSNs in ArcTRAP mice. C , Following viral infusion, mice underwent ∼8 weeks of operant alcohol self-administration, with tagging occurred during the last two days of the training. Two weeks later, they underwent daily chemogenetic activation (via C21 injection, 1 mg/kg) during extinction sessions, followed by cue-induced reinstatement testing without C21 injection. D , Active lever pressing during acquisition was comparable between groups. Two-way RM ANOVA: F (1, 11) = 0.29, p = 0.60. E , Chemogenetic activation of acquisition-recruited dMSNs resulted in persistently elevated responding across extinction sessions. Two-way RM ANOVA: F (1, 11) = 5.697, # p = 0.036. F , Activation of acquisition-recruited dMSNs during extinction enhanced active presses during cue-induced reinstatement. Unpaired t test: t (11) = 2.46, *p = 0.032. G , Reinstatement responding expressed as a percentage of acquisition responding demonstrates that activation of acquisition ensembles during extinction promotes relapse. Unpaired t test: t (11) = 2.59, *p = 0.025. n = 7 mice (mCh) and 6 mice (Gq).

Journal: bioRxiv

Article Title: Extinction Training Suppresses Alcohol Relapse by Inhibiting Acquisition-Recruited Striatal Ensembles and Engaging Striosomal Neurons

doi: 10.64898/2026.04.30.722024

Figure Lengend Snippet: A , During extinction, acquisition-recruited striatal ensembles are suppressed, contributing to the reduction of alcohol-seeking behavior. We hypothesized that chemogenetic activation of these acquisition-recruited neurons during extinction would counteract this suppression, impair extinction learning, and promote relapse. B , Schematic illustrating intersectional viral approach to express DREADD in acquisition-recruited striatal dMSNs in ArcTRAP mice. C , Following viral infusion, mice underwent ∼8 weeks of operant alcohol self-administration, with tagging occurred during the last two days of the training. Two weeks later, they underwent daily chemogenetic activation (via C21 injection, 1 mg/kg) during extinction sessions, followed by cue-induced reinstatement testing without C21 injection. D , Active lever pressing during acquisition was comparable between groups. Two-way RM ANOVA: F (1, 11) = 0.29, p = 0.60. E , Chemogenetic activation of acquisition-recruited dMSNs resulted in persistently elevated responding across extinction sessions. Two-way RM ANOVA: F (1, 11) = 5.697, # p = 0.036. F , Activation of acquisition-recruited dMSNs during extinction enhanced active presses during cue-induced reinstatement. Unpaired t test: t (11) = 2.46, *p = 0.032. G , Reinstatement responding expressed as a percentage of acquisition responding demonstrates that activation of acquisition ensembles during extinction promotes relapse. Unpaired t test: t (11) = 2.59, *p = 0.025. n = 7 mice (mCh) and 6 mice (Gq).

Article Snippet: Drd1a-tdTomato (#016204), Snap25-LSL-F2A-GFP (#021879), FosTRAP (Fos 2A-iCreER ; #030323), and ArcTRAP (Arc CreER ; #021881) , , mice were purchased from the Jackson Laboratory.

Techniques: Activation Assay, Injection